Peptide Protocol
Peptide Protocol
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Semaglutide peptide calculator

Pre-filled Semaglutide dosage and reconstitution math: a 5 mg vial, 2 ml of bacteriostatic water, and the resulting U-100 insulin-syringe units. Adjust the inputs below or open the full calculator.

Typical 250 mcg dose:
units on a 100u insulin syringe
= ml per injection · concentration mg/ml
Open full calculator with these values →

How to reconstitute Semaglutide

  1. Wipe both vial stoppers with a fresh alcohol swab and let them dry.
  2. Draw 2 ml of bacteriostatic water into a syringe.
  3. Insert the needle at an angle into the Semaglutide vial and let the water run slowly down the inner wall — do not inject directly onto the powder.
  4. Do not shake. Gently swirl the vial until the peptide fully dissolves.
  5. Store the reconstituted vial refrigerated (2–8 °C / 36–46 °F) and use within roughly 28 days.
  6. Draw each dose to the unit mark shown above for your target 250 mcg dose.

About Semaglutide

Semaglutide is a long-acting GLP-1 receptor agonist approved for type 2 diabetes and chronic weight management. It slows gastric emptying, enhances insulin secretion, and reduces appetite.

Half-life: Approximately 7 days (once-weekly dosing)
Typical dose range: Titration: 0.25 mg (250 mcg) → 0.5 mg → 1.0 mg → 1.7 mg → 2.4 mg weekly
Class: GLP-1 receptor agonist

Read the full Semaglutide guide →

Semaglutide calculator FAQ

Is this a Semaglutide peptide calculator or a reconstitution calculator?

Both. This Semaglutide calculator converts vial strength, bacteriostatic-water volume, and target dose into milliliters and insulin-syringe units. It does not recommend a medical dose.

How much bacteriostatic water for a 5 mg Semaglutide vial?

This page is pre-filled with 2 ml of bacteriostatic water for a 5 mg Semaglutide vial because that is a common research ratio. You can adjust the water amount in the full calculator.

How many units is a 250 mcg Semaglutide dose?

With the default 5 mg vial and 2 ml BAC-water ratio, the preview above calculates the U-100 syringe units for a 250 mcg dose. Change the dose if your protocol uses a different amount.

Does this Semaglutide dosage calculator give medical advice?

No. It only performs unit math. Dosing, titration, and whether Semaglutide is appropriate are clinical decisions that should be made with a licensed healthcare professional.

Skip the math — let the app handle it

Peptide Protocol pre-fills reconstitution, tracks every dose, and flags rotations automatically.

See the app →

Educational use only. Peptide Protocol is an informational tool. Nothing on this page constitutes medical advice. Many peptides are prescription-only or restricted in your jurisdiction. Always consult a licensed healthcare professional before injecting any compound.