Peptide Protocol
Peptide Protocol
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TB-500 peptide calculator

Pre-filled TB-500 dosage and reconstitution math: a 10 mg vial, 3 ml of bacteriostatic water, and the resulting U-100 insulin-syringe units. Adjust the inputs below or open the full calculator.

Typical 2000 mcg dose:
units on a 100u insulin syringe
= ml per injection · concentration mg/ml
Open full calculator with these values →

How to reconstitute TB-500

  1. Wipe both vial stoppers with a fresh alcohol swab and let them dry.
  2. Draw 3 ml of bacteriostatic water into a syringe.
  3. Insert the needle at an angle into the TB-500 vial and let the water run slowly down the inner wall — do not inject directly onto the powder.
  4. Do not shake. Gently swirl the vial until the peptide fully dissolves.
  5. Store the reconstituted vial refrigerated (2–8 °C / 36–46 °F) and use within roughly 28 days.
  6. Draw each dose to the unit mark shown above for your target 2000 mcg dose.

About TB-500

TB-500 is a synthetic peptide fragment based on the active region of thymosin beta-4, a naturally occurring protein involved in cell migration, angiogenesis, and wound healing.

Half-life: Approximately 2 hours (peptide); biological effect lasts longer
Typical dose range: 2–5 mg (2000–5000 mcg) per injection, 1–2x weekly loading, then maintenance
Class: Synthetic peptide fragment of thymosin beta-4

Read the full TB-500 guide →

TB-500 calculator FAQ

Is this a TB-500 peptide calculator or a reconstitution calculator?

Both. This TB-500 calculator converts vial strength, bacteriostatic-water volume, and target dose into milliliters and insulin-syringe units. It does not recommend a medical dose.

How much bacteriostatic water for a 10 mg TB-500 vial?

This page is pre-filled with 3 ml of bacteriostatic water for a 10 mg TB-500 vial because that is a common research ratio. You can adjust the water amount in the full calculator.

How many units is a 2000 mcg TB-500 dose?

With the default 10 mg vial and 3 ml BAC-water ratio, the preview above calculates the U-100 syringe units for a 2000 mcg dose. Change the dose if your protocol uses a different amount.

Does this TB-500 dosage calculator give medical advice?

No. It only performs unit math. Dosing, titration, and whether TB-500 is appropriate are clinical decisions that should be made with a licensed healthcare professional.

Skip the math — let the app handle it

Peptide Protocol pre-fills reconstitution, tracks every dose, and flags rotations automatically.

See the app →

Educational use only. Peptide Protocol is an informational tool. Nothing on this page constitutes medical advice. Many peptides are prescription-only or restricted in your jurisdiction. Always consult a licensed healthcare professional before injecting any compound.